Year: 2014 | Month: July | Volume 7 | Special Issue
Bioremediation potential of Comamonas acidovorans MTCC 3364 for the removal of sulfonated di-azo dye Reactive Black B
Abstract:
Azo dyes are a chief class of synthetic colorants, which are released by the majority of the textile industries. The effluents of dyes disrupt the ecosystem so removal of these dyes is major concerned by using cost-competitive and eco-friendly method. The present study was aimed to study the decolorization efficiency of the textile azo dyes by Comamonas acidovorans MTCC 3364 and optimize the environmental condition for maximum decolorization and degradation of Reactive Black B (RBB) dye. Optimization of various environmental parameters like pH and temperature was studied in which maximum decolorization was obtained at 37°C, pH 7.0 under static condition within 24 hours. The addition of co-substrates lactose and yeast extract increased the rate of decolorization. The bacterial strain was able to decolorize high concentration of RBB dye (1 g l-1) up to 8th cycle. Vanillin was added as a redox mediator which showed the highest rate of decolorization (1.062 mg l-1 h-1) and thiourea was added as an inhibitor, which showed highest inhibition (0.246 mg l-1 h-1). Incubation of dye with a non-growing (free) cells and dead cells resulted in removal of dye from the buffer, indicating the biosorption and adsorption mechanism. Immobilization cell studies revealed that activated immobilized cell preparations decolorized RBB dye up to 10 cycles showing remarkable operational stability. The degradation analysis of RBB was further confirmed by HPTLC and FTIR techniques.
© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Print This Article Email This Article to Your Friend
